Profile of key immune-related genes in penaeus monodon juveniles after oral administration of recombinant envelope protein vp28 of white spot syndrome virus, microbial pathogenesis (2016), doi: 101016/jmicpath201605002 this is a pdf file of an unedited manuscript that has been accepted for publication. Systems have been experimented, such as oral administration of peptidoglycan, lipopolysacharides, β-1,3 glucan (itami et al, 1998 takahashi et al, 2000 chang et al, 2003), vaccination with inactivated viral preparation and viral envelop protein vp19 and vp 28 (singh et al, 2005 namikoshi et al, 2004. Oral administration is a route of administration where a substance is taken through the mouth per os (po) is sometimes used as an abbreviation for medication to be taken orally many medications are taken orally because they are intended to have a systemic effect, reaching different parts of the body via the bloodstream,. This indicated that oral administration of vp28-transformed algae can protect crayfish against viral pathogens staphylococcus aureus is a bacterium that can cause skin infections, respiratory diseases and food poisoning the fused protein of d2 fibronectin-binding domain of s aureus and the ctb. Envelope protein, vp28 this suggests the potential use of pmrab7 for wssv protection in shrimp aquaculture management the pichia pastoris expression system was used in pmrab7 production for farm trials by oral administration in the field trial, 1 mg and 2 mg pmrab7/kg shrimp feed were used for wssv protection. Vp39 protein against wssv infection in litopenaeus vannamei by intramuscular and oral administration, and also compared the efficacy with recombinant vp28 ( rvp28) the vp39 is a 283 amino acid protein encoded by the structural gene vp39 which acts as an important mediator for virus entry to the host shrimp orally. Vp28 the adjuvant effects of poly i:c and imiquimod during immunization with a recombinant protein, rvp28 derived from white spot syndrome virus (wssv) was investigated in recently, the protective efficacy of oral delivery and injection of a drome disease after oral administration of recombinant vp28 expressed in.
Using rnai technology by oral administration of bacterially expressed dsrna of the wssv vp28 gene materials and methods preparation of viral inoculum viral inoculum was prepared from wssv-infected penaeus monodon with prominent white spots collected from shrimp farms located near nellore,. Disease after oral administration of recombinant vp28 expressed in brevibacillus brevis, fish and shellfish immunology, 2008, 25 (3), 315-320 15 fu, ll, li, wf, du, hh, dai, w, xu, zr: oral vaccination with envelope protein vp28 against white spot syndrome virus in procambarus clarkii using bacillus subtilis as. Oral vaccination of baculovirus-expressed vp28 displays enhanced protection against white spot syndrome virus in penaeus monodon administration, oral animals baculoviridae/genetics blotting, western immunoenzyme techniques penaeidae/immunology penaeidae/virology rna,. Spot disease by oral administration of inactivated white spot syndrome virus i s bright singh1,, m can be protected from wsd by simple oral administration of ivp key words: penaeus indicus penaeid shrimp (2004a,b) reported similar results using 2 wssv envelope proteins, vp19 and vp28.
Immunogenic response and are considered for oral administration [mauriello et al, 2004] the efficiency of a vaccine can also be increased to some extent as with vp28, the white spot syndrome virus envelope protein, which is considered to be an effective prophylactic vaccine against white shrimp white. Commercial feeds that were mixed with recombinant vp28, a structural protein antigen of wssv for a period of 14 days the immune response of the shrimp during oral administration of the medicated feed was determined by expression analysis of fortilin, a gene that is involved in the antiviral response. Over the past few decades the reliance on cultured shrimp for food production has increased greatly from 1970 to 2006, production of cultivated shrimp increased 350-fold, while shrimp yields from capture fisheries increased only 3- fold  by the end of this period, yields from both industries were close to parity [ 1] despite.
That were mixed with recombinant vp28, a structural protein antigen of wssv for a period of 14 days the immune response of shrimp during oral administration of medicated feed was determined by expression analysis of fortilin, a gene that is involved in the antiviral response there was a significant increase in the level of. The potential of oral administration of wssv envelope proteins to procambarus clarkii crayfish for protection against white spot syndrome virus proteins used included vp28 and vp19 expressed in silkworm using a baculovirus (hynpv) expression system in order to gain correctly folded proteins with biological activity. Oral vaccination with envelope protein vp28 against white spot syndrome virus in procambarus clarkii aims: to achieve high-level expression and secretion of active vp28 directed by a processing-efficient signal peptide in and rvp28-bs was performed by oral administration three groups were challenged at different.
66:265-270 caipang cma, verjan n, ooi el, kondo h, hirono i, aoki t, kiyono h, yuki y (2008) enhanced survival of shrimp, penaeus (marsupenaeus) japonicus from white spot syndrome disease after oral administration of recombinant vp28 expressed in brevibacillus brevis fish & shellfish immunology 25:315-320. 27 tháng 4 2015 spot syndrome virus infection in penaeus monodon by oral administration of vp28- bacillus subtilis từ khóa: tôm sú, bacillus subtilis vp28, đáp ứng miễn dịch keywords: penaeus monodon, bacillus subtilis, vp28, immune response abstract recombinant vp28 (rvp28) of wssv has been shown.
The target viral genes in this study include a major structural protein (vp28) and a hub protein (wssv051) vp28 is involved in the viral entry to shrimp cells, and injection of dsrna corresponding to vp28 was shown to effect- ively protect shrimp against the virus [11, 13, 14] oral administration of vp28-specific dsrna was. In this study, l vannamei shrimp were orally vaccinated with inactivated bacteria over expressing vp28 and challenged with wssv by immersion the results an explanation for this discrepancy could lie in the differences in administration of the vaccine and wssv challenge used in both vaccination methods vp19. Oral vaccination with envelope protein vp28 against white spot syndrome virus in procambarus clarkii using bacillus subtilis as delivery vehicles the comparative effect potential on groups of rvp28-bv and rvp28-bs was performed by oral administration three groups were challenged at different time.